Dr. Stephen Baron
Degree Award Date
DNA Fingerprinting, Alu TPA-25 Locus, Humans, Amersham-Pharmacia Biotech Ready-to-Go Bead system, pedigree chart
Biology | Genetics and Genomics | Laboratory and Basic Science Research | Molecular Genetics
The goal of this research was to determine the Alu TPA-25 DNA fingerprint of my family members in order to construct a genetic pedigree. A non-invasive techniq ue was used to acquire buccal cells from 9 experimental subjects. Genomic DNA was released from the cells, mixed with specific primers, and subjected to the polymerase chain reaction (PCR) in order to amplify the Alu TP A-25 locus of chromosome 8. The PCR products were then electrophoresed on a 1.5% agarose gel to reveal the subject's genotype with respect to the Alu TPA-25 element.
Optimal amounts of PCR product were obtained by using the Amersham-Pharmacia Biotech Ready-to-Go Bead® system. Results with this system showed that I am homozygous recessive for the Alu TP A-25 allele. Similarly, all of my family members were also homozygous recessive. I used this information to create a pedigree chart of the Alu TPA-25 genotypes for my family.
Carlson, Jocelyn R., "DNA Fingerprinting of the Alu TPA-25 Locus in Humans" (2001). Honors Projects. 437.