Document Type

Honors Project

First Advisor

Dr. Stephen Baron

Degree Award Date

Spring 2001

Keywords

DNA Fingerprinting, Alu TPA-25 Locus, Humans, Amersham-Pharmacia Biotech Ready-to-Go Bead system, pedigree chart

Disciplines

Biology | Genetics and Genomics | Laboratory and Basic Science Research | Molecular Genetics

Abstract

The goal of this research was to determine the Alu TPA-25 DNA fingerprint of my family members in order to construct a genetic pedigree. A non-invasive techniq ue was used to acquire buccal cells from 9 experimental subjects. Genomic DNA was released from the cells, mixed with specific primers, and subjected to the polymerase chain reaction (PCR) in order to amplify the Alu TP A-25 locus of chromosome 8. The PCR products were then electrophoresed on a 1.5% agarose gel to reveal the subject's genotype with respect to the Alu TPA-25 element.

Optimal amounts of PCR product were obtained by using the Amersham-Pharmacia Biotech Ready-to-Go Bead® system. Results with this system showed that I am homozygous recessive for the Alu TP A-25 allele. Similarly, all of my family members were also homozygous recessive. I used this information to create a pedigree chart of the Alu TPA-25 genotypes for my family.

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