Document Type

Honors Project

First Advisor

Dr. Stephen Baron

Degree Award Date

Fall 2011


DNA Binding Proteins, Polyhydroxybutyrate Depolymerase Synthesis, Streptomyces sp. 5A.


Bacteriology | Biology | Cell Biology | Microbiology


The overall goal of the research was to identify DNA-binding proteins that might be involved in transcriptional regulation of phaZ. Specific goals were the following: 1) to construct DNA probes for use in EMSA; 2) to produce cell extracts of Streptomyces sp. 5A grown under inducing and repressing conditions; 3) to perform the EMSA technique to identify DNA-protein complexes. All goals were met and the EMSA technique was conducted three times. In the third EMSA procedure a possible shift was noted in PHB cell extracts collected after 29 hours of incubation.