Dr. Stephen Baron
Degree Award Date
DNA Binding Proteins, Polyhydroxybutyrate Depolymerase Synthesis, Streptomyces sp. 5A.
Bacteriology | Biology | Cell Biology | Microbiology
The overall goal of the research was to identify DNA-binding proteins that might be involved in transcriptional regulation of phaZ. Specific goals were the following: 1) to construct DNA probes for use in EMSA; 2) to produce cell extracts of Streptomyces sp. 5A grown under inducing and repressing conditions; 3) to perform the EMSA technique to identify DNA-protein complexes. All goals were met and the EMSA technique was conducted three times. In the third EMSA procedure a possible shift was noted in PHB cell extracts collected after 29 hours of incubation.
Darragh, Amber S., "Detection of DNA Binding Proteins Involved in Regulation of Polyhydroxybutyrate Depolymerase Synthesis in Streptomyces sp. 5A." (2011). Honors Projects. 78.