Dr. Stephen Baron
Dr. Douglas Dennis
Degree Award Date
Cloning, Screening, Polyhydroxyalkanoate Depolymerase, Streptomyces spp. 5A
Biology | Laboratory and Basic Science Research | Molecular Genetics
Polyhydroxyalkanoates (PHAs) are organic polymers produced by some soil bacteria as a carbon source for growth and energy. These PHAs are degraded by a family of enzymes called PHA-depolymerase, which are produced by many species of soil and water bacteria . These bacteria produce PHA-depolymerase and then use the degraded PHA as a source of carbon for energy and growth. This study will deal specifically with the PHA-depolymerase produced by Streptomyces spp. 5A.
The goal of the study was to create a partial gene library of the Streptomyces spp. 5A genomic DNA and then clone that library into Eschericia coli using the plasmid vector pBluescript SKII. Once created, the hbrary was screened for PHA-depolymerase production using selective PHA agar plates and oligonucleotide probes in order to find the desired gene.
Rowe, Sean A., "Cloning and Screening of the Gene for Polyhydroxyalkanoate Depolymerase from Streptomyces spp. 5A" (2003). Honors Projects. 369.