Authors

Ashley Crossman

Document Type

Honors Project

First Advisor

Dr. Stephen Baron

Degree Award Date

Spring 2016

Keywords

Streptomyces sp. 5A, Bacteriophages, Purification, Genomic Sequencing

Disciplines

Biology | Biotechnology | Genetics and Genomics | Genomics | Immunology and Infectious Disease

Abstract

The isolation, sequencing, and study of the bacteriophages which target this genus, may yield invaluable insight into the gene expression, regulation, and population evolution of Streptomyces phages. This research could not only contribute to the development of molecular tools in Streptomyces research, but also in biotechnology and within the medical field. The immediate goal of this research was to isolate bacteriophages of Streptomyces sp. 5A and purify their DNA. The longer term goal was to sequence the DNA and locate genes for PGH's which could be cloned and overexpressed in Escherichia coli for therapeutic use.

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