Maria Marlin

Document Type

Honors Project

First Advisor

Dr. Edgar Lickey

Degree Award Date

Spring 2016

Keywords

Chlorosplenium chlora (Schwein.) M.A. Curtis, saprobic ascomycete

Disciplines

Biology | Genetics and Genomics

Abstract

Chlorosplenium chlora (Schwein.) M.A. Curtis is a saprobic ascomycete that occurs on the underside of fallen hardwood branches. This fungus, while discovered in 1822, has received very little attention; sequences of its DNA have not been reported, and its mating behavior and ecology have not been researched. The more familiar green-stain fungus, Chlorociboria Fries, is a genus possessing very similar morphological characteristics and was once considered a close relative of Chlorosplenium. Macroscopic characters of Chlorosplenium chlora, including ascocarp size and color were analyzed as well as the number and density of fruit bodies and potential substrate preference. Microscopic characters, including ascospore size and shape and asci size are also reported. Additionally, genetic differences between geographically isolated populations were investigated using nuclear ribosomal ITS 1 - 5.8S - ITS 2, and C. chlora 's phylogenetic relationships with its fellow Leotiomycete relatives were analyzed using nuclear ribosomal Large Subunit DNA sequences.

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