Dr. Moshe Khurgel
Degree Award Date
Reinnervation, limb regeneration, axolotl, Ambystoma mexicanum
Animal Sciences | Biology
Nine experimental axolotls (Ambystoma mexicanum) of the same age ranging from 191 - 202 mm in total length and 52.3 - 59.5 g in weight were used (Table 1 ). Three additional axolotls were used as immunohistochemical and histological controls: Two received no injections and remained unamputated to serve as negative immunohistochemical and histological controls; the other axolotl received multiple injections and underwent amputation to serve as a positive immunohistochemical and histological control. One additional axolotl was used at a later date to test an alternate surgical procedure for neuronal tracing. All axolotls were housed in 1X Holtfreter's solution maintained at room temperature on a 12:12 hours light/dark cycle. Additionally, all axolotls had undergone amputations of anterior and posterior limbs on the right side approximately one year prior to the beginning of this study. All four limbs on each of the nine experimental animals were measured before amputations to provide a long-term method for tracking their regeneration. Axolotls were anesthetized in 3:7 10X benzocaine (Sigma):1X Holtfreter's solution for all surg,ical procedures.
Wright, Kristen Ashley, "Reinnervation during limb regeneration in the axolotl (Ambystoma mexicanum)" (2011). Honors Projects. 99.