Document Type
Honors Project
First Advisor
Dr. Edgar Lickey
Degree Award Date
Spring 2012
Disciplines
Biology | Pharmacology, Toxicology and Environmental Health
Abstract
In this study, the antibacterial and antifungal activities of several basidiomycete species were analyzed using extracts of dried material and/or liquid GPY growth media (glucose, peptone, yeast extract) of live cultures. The species surveyed include Armillaria mellea, Agaricus sylvicola, Agaricus arvensis, Lepista nuda, and Lactarius vinaceorufescens among others. Intracellular metabolites were obtained from dried fungal specimens and were extracted using a Soxhlet extractor. These metabolites were then dissolved into a set of three different solvents: ethyl acetate, methanol, and chloroform. Extracellular metabolites were obtained from the GPY liquid medium after approximately 6 to 8 weeks of mycelial growth. These metabolites were filtered to isolate compounds under 10,000 MW. All extracts were tested against seven bacteria: Staphylococcus aureus, S. epidermidis, Salmonella typhimurium, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilus, and Enterobacter aerogenes. Intracellular extracts from dried material of Agaricus arvensis showed antibiotic activity against B. subtilus at a concentration of 30 g/L when dissolved in methanol.
Recommended Citation
Propst, Lindsey, "Antibiotic Activity of Extracts of Several Basidiomycete Species" (2012). Honors Projects. 95.
https://digitalcommons.bridgewater.edu/honors_projects/95