Lindsey Propst

Document Type

Honors Project

First Advisor

Dr. Edgar Lickey

Degree Award Date

Spring 2012


Biology | Pharmacology, Toxicology and Environmental Health


In this study, the antibacterial and antifungal activities of several basidiomycete species were analyzed using extracts of dried material and/or liquid GPY growth media (glucose, peptone, yeast extract) of live cultures. The species surveyed include Armillaria mellea, Agaricus sylvicola, Agaricus arvensis, Lepista nuda, and Lactarius vinaceorufescens among others. Intracellular metabolites were obtained from dried fungal specimens and were extracted using a Soxhlet extractor. These metabolites were then dissolved into a set of three different solvents: ethyl acetate, methanol, and chloroform. Extracellular metabolites were obtained from the GPY liquid medium after approximately 6 to 8 weeks of mycelial growth. These metabolites were filtered to isolate compounds under 10,000 MW. All extracts were tested against seven bacteria: Staphylococcus aureus, S. epidermidis, Salmonella typhimurium, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilus, and Enterobacter aerogenes. Intracellular extracts from dried material of Agaricus arvensis showed antibiotic activity against B. subtilus at a concentration of 30 g/L when dissolved in methanol.