Document Type

Honors Project

First Advisor

Dr. Robert Shute

Second Advisor

Dr. Leah Washington

Third Advisor

Dr. Charles Hale

Degree Award Date

Spring 5-2025

Keywords

exercise physiology, testosterone, cortisol, hormones, cycling

Disciplines

Kinesiology | Medical Sciences | Medicine and Health Sciences | Physiology

Abstract

Testosterone is an anabolic-androgenic steroid released from the gonads when exercising. Additionally, cortisol is a catabolic hormone that is also released in response to the stress of exercise. Both hormones have been known to increase significantly following high-intensity exercise as compared to low-intensity exercise. However, more research needs to be done regarding the interval training effect on testosterone and cortisol levels. PURPOSE: The purpose of this study is to determine the hormonal response of testosterone and cortisol during interval versus constant load cycling. METHODS: Participants aged 19-45 years old will be recruited to participate in the study. Their initial visit will consist of performing a cycle ergometer VO2max test until failure. The next two visits are experimental trials where participants will perform a constant load and interval load cycling test. The interval load test will be a 30-minute cycle made up of 3-minute intervals between 40 and 80% of the participants VO2max. The constant load test will be a 30-minute trial at 60% of the participants VO2max for the entirety of the time. Before and after both experimental trials, saliva will be collected to analyze testosterone and cortisol concentrations using an enzyme-linked immunosorbent assay (ELISA). IMPLICATIONS: This study will provide insight regarding testosterone and cortisol’s response to exercise at various intensities. Understanding what intensities result in a greater increase of these hormones can enable the creation of exercise programs that promote muscle growth.

Recommended Citation

Zurlo, Anna. "The Effects of Interval and Constant Load Cycling on Cortisol and Testosterone Production.” Senior Honors Projects, Bridgewater College, 2025.

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