Dr. Joseph Crockett
Degree Award Date
Enzyme Inhibition, Acid Phosphatase, Lineweaver-Burke plot, Hanes plot
Analytical Chemistry | Chemistry | Laboratory and Basic Science Research
The purpose of this experiment was to study the reaction of acid phosphatase with p-nitrophenyl phosphate (PNPP) substrate to yield p-nitrophenol (PNP) and a free phosphate. Km and V max values of the reaction were determined using the Lineweaver-Burke plot and the Hanes plot. Various inorganic anion and organic acid inhibitors were added, and Km and V max values were compared to those of the original reaction in order to determine the type of inhibition occurring.
McClelland, Marcy L., "Enzyme Inhibition of Acid Phosphatase" (2001). Honors Projects. 440.