Document Type

Honors Project

Publication Date

Spring 2025

Abstract

Testosterone is an anabolic-androgenic steroid released from the gonads when exercising. Additionally, cortisol is a catabolic hormone that is also released in response to the stress of exercise. Both hormones have been known to increase significantly following high-intensity exercise as compared to low-intensity exercise. However, more research needs to be done regarding the interval training effect on testosterone and cortisol levels. PURPOSE: The purpose of this study is to determine the hormonal response of testosterone and cortisol during interval versus constant load cycling. METHODS: Participants aged 19-45 years old will be recruited to participate in the study. Their initial visit will consist of performing a cycle ergometer VO2max test until failure. The next two visits are experimental trials where participants will perform a constant load and interval load cycling test. The interval load test will be a 30-minute cycle made up of 3-minute intervals between 40 and 80% of the participants VO2max. The constant load test will be a 30-minute trial at 60% of the participants VO2max for the entirety of the time. Before and after both experimental trials, saliva will be collected to analyze testosterone and cortisol concentrations using an enzyme-linked immunosorbent assay (ELISA). IMPLICATIONS: This study will provide insight regarding testosterone and cortisol’s response to exercise at various intensities. Understanding what intensities result in a greater increase of these hormones can enable the creation of exercise programs that promote muscle growth.

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